Virgin Beta Cells Persist throughout Life at a Neogenic Niche within Pancreatic Islets
Virtual slicing of a 3D reconstructed islet from a mouse carrying Ins1-H2b-mCherry as well as Ucn3-eGFP.
Fluorescent glucose uptake in an islet over time.
Streptozotocin-mediated death of mature beta cells spares immature beta cells at the islet edge.
Visualization of alpha to beta trans-differentiated cells at the periphery of an Ins1-H2bmCherry x Gcg-Cre x lsl-eYFP triple transgenic islet.
Stereotypical alpha cell calcium activity measured by GCaMP6 upon AVP stimulation followed by stereotypical beta cell GCaMP6 activity upon continued high glucose stimulation.
Alpha-to-beta transdifferentiated cell calcium (GCaMP6) activity is indistinguishable from that of beta cells.
Beta-to-alpha transdifferentiated cell calcium (GCaMP6) activity is indistinguishable from that of alpha cells.
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van der Meulen T, Mawla AM, DiGruccio MR, Adams MW, Nies V, Dölleman S, Liu S, Ackermann AM, Cáceres E, Hunter AE, Kaestner KH, Donaldson CJ, Huising MO (2017). Virgin Beta Cells Persist throughout Life at a Neogenic Niche within Pancreatic Islets. Cell Metabolism, 25(4): 911-926.